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Nathaniel R. Kinsky*, Mihály Vöröslakos, Jose Roberto Lopez Ruiz, Laurel Watkins de Jong, Nathan Slager, Sam McKenzie, Euisik Yoon, and Kamran Diba*
Simultaneous Electrophysiology and Optogenetic Perturbation of the Same Neurons in Chronically Implanted Animals Using μLED Silicon Probes
STAR Protoc., 4 (4), 102570
Date: Sep 19, 2023

Micro-light-emitting-diode (μLED) silicon probes feature independently controllable miniature light-emitting-diodes (LEDs) embedded at several positions in each shank of a multi-shank probe, enabling temporally and spatially precise optogenetic neural circuit interrogation. Here, we present a protocol for performing causal and reproducible neural circuit manipulations in chronically implanted, freely moving animals. We describe steps for introducing optogenetic constructs, preparing and implanting a μLED probe, performing simultaneous in vivo electrophysiology with focal optogenetic perturbation, and recovering a probe following termination of an experiment.

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